ICSI Injection and Holding Pipettes
Description
ICSI Injection and Holding Pipettes are professional-grade glass micro-tools engineered from premium borosilicate glass for high-precision micromanipulation during Intracytoplasmic Sperm Injection (ICSI). The collection features ultra-sharp injection needles designed to smoothly penetrate the oocyte membrane alongside flame-polished holding pipettes engineered to firmly anchor the specimen via gentle suction. Individually validated and tested, these instruments ensure minimal physical deformation and maximum gamete safety during advanced assisted reproduction procedures.- Our premium series of ICSI Injection and Holding Pipettes represents a comprehensive micromanipulation system tailored to meet the strict mechanical requirements of modern embryology cleanrooms. Achieving a successful fertilization via Intracytoplasmic Sperm Injection demands an intricate balance between secure cellular immobilization and ultra-fine membrane penetration. These specialized instruments work in tandem on micromanipulator stages to give the operator unparalleled tactile feedback and volumetric control.
- The system comprises two core components outlined in the technical documentation.
- The injection pipette is a high-precision micro-needle explicitly designed for the precise aspiration and intracytoplasmic injection of a single motile sperm into a mature metaphase two oocyte during ICSI procedures. Crafted from high-grade borosilicate glass, it features:
- Advanced Tip Geometry: An ultra-sharp bevel configured with customized spike or spike-less options to smoothly pierce the oocyte membrane while minimizing physical deformation.
- Controlled Internal Lumens: An inner diameter calibrated between four point zero micrometer to five point five micrometer to facilitate the smooth aspiration of a single spermatozoon while dramatically minimizing backflow within the line.
- Ergonomic Bending Profiles: Available in standard twenty degree, thirty degree, and thirty-five degree customized variants to adapt flawlessly to different microscope configurations.
- The holding pipette is a specialized glass micro-tool engineered to anchor and stabilize the oocyte or embryo firmly in place during high-stakes injections, biopsy sampling, or assisted hatching procedures. Its characteristics include:
- Ultra-Smooth Tip Finish: Features a broad, flat, flame-polished opening that creates a reliable, tight seal via gentle suction without causing cellular deformation or stress.
- Proportional Architecture: Built with a generous outer-to-inner diameter ratio to keep the biological specimen completely stationary under micro-lateral forces.
- Versatile Sizing and Alignment: Supplied in outer diameter options ranging from eighty micrometer to one hundred and twenty micrometer and inner diameter profiles of fifteen micrometer to twenty-five micrometer, with selectable bending angles from zero degree to thirty-five degree.
- To ensure complete biological compatibility and non-toxicity, every production lot is individually validated and strictly Mouse Embryo Assay (MEA) and Human Sperm Survival Assay (HSSA) tested before cleanroom packaging.
Product Category:
Consumables
- High Grade Pure Borosilicate Glass Micro Tool Architecture.
- Sharp Bevel Injection Needle with Customized Spike Options.
- Ultra Smooth Flame Polished Holding Opening To Prevent Deformation.
- Strictly Validated Mouse Embryo Assay and Human Sperm Survival Assay Controls.
- Generous Outer to Inner Diameter Ratio For Reliable Suction Seals.
- Pre Bent Angle Formats Ranging From Zero to Thirty Five Degrees.
- Atraumatic Membrane Penetration: The spike-tipped injection bevel slices cleanly through the oocyte coat, protecting cell viability and minimizing post-injection lysis.
- Deformation-Free Stabilization: The flame-polished face of the holding pipette secures fragile specimens comfortably without pinching or distorting cellular structure.
- Premium Borosilicate Composition: Constructed entirely from high-grade borosilicate glass to maximize structural stiffness and deliver a distortion-free optical path under high-power contrast microscopy.
- Anti-Backflow Precision: Carefully constrained injection lumens from four point zero micrometer to five point five micrometer provide predictable microfluidic dynamics for single sperm control.
- Comprehensive Clinical Assays: Extensively batch-validated through dual Mouse Embryo Assay and Human Sperm Survival Assay testing protocols to guarantee maximum safety for human gametes and embryos.
- Flexible Angular Customization: Pre-bent configurations perfectly match standard micromanipulator tool holders, bypassing the need for manual adjustment.
- Injection Pipette Specifications
- Material: High-grade Borosilicate Glass
- Inner Diameter (ID): Four point zero micrometer to five point five micrometer
- Tip Geometry: Sharp bevel with customized spike or spike-less options
- Bending Angles: Twenty degree, thirty degree, and thirty-five degree customized variants
- Biocompatibility: Individually validated, Mouse Embryo Assay (MEA) and Human Sperm Survival Assay (HSSA) tested
- Holding Pipette Specifications
- Material: Precision Borosilicate Glass
- Outer Diameter (OD): Eighty micrometer to one hundred and twenty micrometer options
- Inner Diameter (ID): Fifteen micrometer to twenty-five micrometer options
- Tip Finish: Ultra-smooth flame polished edge
- Bending Angle: Available from zero degree to thirty-five degree
- Primary Applications: Anchoring and stabilizing the oocyte or embryo in place during ICSI injections, biopsy sampling, or assisted hatching procedures
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